Source data for: PPP1R3G-RIPK1-ZBP1 Axis Activates Early-stage Apoptosis and Late-stage Necroptosis to Promote Doxorubicin-Induced Cardiotoxicity

This repository contains source data supporting the manuscript: "PPP1R3G-RIPK1-ZBP1 Axis Activates Early-stage Apoptosis and Late-stage Necroptosis to Promote Doxorubicin-Induced Cardiotoxicity".
This study identifies PPP1R3G as a central regulator of RIPK1 activation and delineates a PPP1R3G–RIPK1–ZBP1 signaling axis that drives doxorubicin (DOX)-induced cardiotoxicity through a biphasic program of early apoptosis and late necroptosis.
Contents:
- figures_source_data: Source data underlying all main figures (Fig. 1–6)
- supplementary_data: Source data for supplementary figures (Fig. S1–S6)
Experimental overview: This study includes both in vitro and in vivo experiments. H9c2 cardiomyoblasts, mouse embryonic fibroblasts (MEFs), and primary cardiomyocytes were used to assess cell death, RIPK1 activation, and ZBP1 signaling. Wild-type and Ppp1r3g knockout mice were subjected to doxorubicin-induced cardiotoxicity. Cardiac injury was evaluated using histology, immunostaining, echocardiography, cytokine analysis, and survival studies.
Data notes: Each file contains the quantitative data used to generate the figures. Statistical analyses were performed using GraphPad Prism.
Funding: This work was supported by the National Institutes of Health (R01GM120502, R01GM147474, R35GM158455) and the American Heart Association (26POST1196141).