Unique Genetic Diversity Patterns and Extreme Endemism in Temperate Springs: Evidence from Narrow-Range Endemic Snails

Datasets and Voucher Photos for the project "Unique Genetic Diversity Patterns and Extreme Endemism in Temperate Springs: Evidence from Narrow-Range Endemic Snails".The folder "Population Datasets and Files" contains complete folders outputted from STACKS v.2.66 for individual species and a complete dataset of all species.Assembly is outlined in section 2.2 in the Materials and Methods.Dataset statistics are included in the Supplementary Material.Each individual species has a multiple SNPs per locus dataset, a single SNP per locus dataset, and a reduced multiple SNPs per locus dataset for demographic analyses.There are also two complete datasets that include the outgroup, Elimia aff. carinifera, One multiple SNPSs per locus and one single SNP per locus dataset.<br>The folder "Concatenated and Species Tree Inference" contains input and output files, as outlined in section 2.3 in the Materials and Methods, for the following programs:ASTRAL-IIIMaximum Likelihood Performed in IQtreeOrientAGraphSNAPPSNAQ<br>The folder "Genetic Diversity" contains output files from STACKS v.2.66 and the program populations for individual species used for Genetic Diversity estimates and input files used for Population Structure analyses (both listed below), as outlined in section 2.4 of the Materials and Methods.Genetic Diversity Statistics = observed heterozygosity (Ho), expected heterozygosity (He), nucleotide diversity (π), and inbreeding coefficients (FIS)Pairwise FST values - Between Species<i>Elimia bellacrenata</i> AMOVA - Population Structure Between Sites.LEA - Genetic AdmixturefineRADstructure - Genetic Coancestry<br>The zipped folder "Demographic Analysis " contains input and output files, parameter files, and R Code for individual species demographic analysis, as outlined in section 2.5 of the Materials and Methods, in the program Fastsimcoal2.The zipped folder "Voucher Photos" contains photos of individual shell vouchers for specimens used in this study.Specimens are deposited at the National Museum of Natural History.Questions or Comments: