OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines

Immune-profiling of T helper 1 or Th1 (IFNg, TNF and IL-2), Th2 (IL-4 and 5) and Th17 (IL-17) signatures in murine splenocytes by targeting intracellular cytokines using spectral flow cytometry.
Conclusion:
Using spectral flow cytometry, we developed a 17-parameter panel to profile major immune cell subsets, memory phenotypes and functional cytokines (Th1/2/17) in murine splenocytes in steady state as well as in stimulated conditions. Our panel could be easily modified, if researchers wish to tailor the panel to their specific needs.
Notes:
https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.24926 Quality control of the ID7000 is performed daily by the DFCI cytometry core using AlignCheck Particles (Sony, Cat# AE700510).