Quantifying yeast lipidomics by high performance thin layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics.

Here we present evidence that high performance thin layer chromatography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.