Auricularia sinodelicata transcriptomic

This study investigated the physiological and molecular adaptations of Auricularia sinodelicata to varying humidity levels (45%, 65%, 85%) through transcriptomic analysis. RNA-seq identified 3,434 differentially expressed genes (DEGs), among which 142 exhibited significant expression shifts across treatments. Under humidity stress, cellular integrity was compromised, as evidenced by reduced organelle density, membrane damage, and cytoplasmic lysis. Concurrently, a robust antioxidant response involving catalase (CAT) and superoxide dismutase (SOD) mitigated ROS-induced oxidative stress. Gene Ontology (GO) enrichment analysis revealed that DEGs were primarily associated with metabolic processes, cellular biosynthesis, and organonitrogen compound metabolism. KEGG analysis highlighted key stress-responsive pathways, including ribosome biogenesis, endoplasmic reticulum protein processing, amino acid/nucleotide sugar metabolism, oxidative phosphorylation, and MAPK/glutathione signaling. Critical DEGs were validated via qPCR. The findings demonstrated that metabolic reprogramming in energy production, amino acid utilization, and redox homeostasis underpinned the fungal adaptation to humidity stress. This work elucidated the molecular mechanisms employed by A. sinodelicata to cope with environmental fluctuations. Furthermore, the study revealed that Auricularia sinodelicata contained a significant number of genes associated with antibiotics, vitamins, and other health-related components. This discovery laid the foundation for subsequent resource development and utilization.