Ilya Ravkin (2011) CIL:32058, Homo sapiens, osteocarcenoma. CIL. Dataset

This image set is of Transfluor assay where an orphan GPCR (G-protein coupled receptor) is stably integrated into a beta-arrestin GFP (green) expressing U2OS cell line also showing nucleus (red). After one hour incubation with a compound, the cells were quenched with fixative (formaldehyde) and the plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. Negative results are a more uniform distribution of GFP throughout the cytoplasm and positive results are a peri-nuclear localization.
Recommended attribution: "We used the SBS Roche Transfluor image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."
Analysis of the data set can be downloaded from http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html

本图像集展示了转氟酶测定实验，其中孤儿G蛋白偶联受体（G-protein coupled receptor，GPCR）稳定整合于表达绿色荧光蛋白（GFP）的β-阻断蛋白的U2OS细胞系中，同时可见细胞核（红色）。经过与化合物一小时孵育后，细胞用固定剂（甲醛）进行淬灭处理，并在Cellomics ArrayScan HCS Reader上使用GPCR生物应用进行读取。阴性结果表现为细胞质中GFP的分布更为均匀，而阳性结果则呈现细胞核周围的定位。