Onion (Allium cepa L.) DHW30006 Genome Information

Onion (<i>Allium cepa</i> L.) is an economically valuable crop, but its large, repeat-enriched genome makes genome assembly difficult and limits molecular breeding and biological studies. Herein, we present a chromosomal-level reference genome assembly of the double-haploid onion line DHW30006, constructed by combining PacBio, Illumina, and Hi-C sequencing approaches. The assembled genome totaled 12.77 Gb, with 65,730 gene models, and was anchored to eight pseudo-chromosomes covering 12.07 Gb (94.5%), with a scaffold N50 of 1.40 Gb. DHW30006 onion genome contained improved gene models covering approximately 580 Mb (4.54%) of the genic regions with an average gene length of 8,827 bp and 5.48 exons per gene. These gene models represented the most improved annotation among <i>Allium</i> genomes. This onion genome will serve as a valuable resource for breeding and biological research in <i>Allium</i> plants.洋葱（*Allium cepa* L.）是一种具有重要经济价值的作物，但其庞大且富含重复序列的基因组给基因组组装带来了极大挑战，同时限制了其分子育种与生物学研究。本研究结合PacBio、Illumina及Hi-C测序技术，构建了双单倍体洋葱品系DHW30006的染色体级参考基因组组装结果。组装得到的基因组总长度为12.77 Gb，共注释得到65730个基因模型；其中94.5%的序列（合计12.07 Gb）被锚定至8条假染色体上，scaffold N50达到1.40 Gb。DHW30006洋葱基因组优化后的基因模型覆盖约580 Mb（4.54%）的基因区域，平均基因长度为8827 bp，单个基因平均包含5.48个外显子。该基因注释模型是葱属（*Allium*）基因组现有注释中质量最优的之一。该洋葱基因组将为葱属植物的育种及生物学研究提供宝贵的基因组资源。