Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [Digenome-seq]

To compare the sensitivity of CHANGE-seq-BE to another leading off-target discovery method, we performed Digenome-seq on the same ABE and CBE targets and evaluated them with matching analysis parameters. Many low-frequency off-target sites with editing rates were not identified by Digenome-seq and were only discovered by CHANGE-seq-BE. Overall design: Digenome-seq applied for ABEs and CBEs targeting B2M, CBLB, CD7, CIITA, and PDCD1 in primary human T-cells