ChIP-seq analyis of SVG-p12 human fetal glial cells with exogenous expression of wildtype SOX10 or schwannoma-derived mutant isoforms

To investigate the chromatin binding patterns of wildtype SOX10 versus schwannoma-derived mutant isoforms, we established SVG-p12 cell lines stably expressing either FLAG epitope tagged wildtype SOX10 or two different mutant isoforms of SOX10. We then performed chromatin immunoprecipitation-DNA sequencing on these SVG-p12 cell lines stably transduced with wildtype or mutant SOX10 isoforms using an anti-FLAG epitope antibody. Comparative chromatin binding analysis of ChIP-seq data for SVG-p12 cells following stable transduction wildtype SOX10 or two different tumor-derived mutant isoforms of SOX10.