CBASS MarHE workshop December, 2024

Short-term acute thermal stress assays using the Coral Bleacing Automated Stress System (CBASS) were used to assay standardized temperature tolerances (ED50s) of Pocillopora verrucosa, Porites sp., and Diploastrea sp. (Voolstra et al. 2020; Evensen et al. 2023; Voolstra et al. 2025; Iakovleva et al. 2024). P. verrucosa and Porites sp. samples were collected at 2 depths (shallow ≤ 4 m and deep ≥ 8m) to assess putative ED50 differences with depth. Diploastrea sp. samples were collected at depths ≥ 7.5m. In addition, benthic reef surveys were conducted following a previously published protocol (Colin et al. 2024) using CoralNet (Beijbom et al. 2015). Benthic reef survey data is available at https://coralnet.ucsd.edu/source/5984/. 

A pilot CBASS run using samples from P. verrucosa and Porites sp. was condcuted to assess optimal CBASS heat-hold temperatures.

Files:

CBASSED50_CBASS_PocPorDip.qmd - R script

PocPorDip_MarHE_EDs_boxplot.pdf - ED50 box plot for species and condition

PocPorDip_MarHE_EDsdf.csv - colony-specific ED5, ED50, ED95

PocPorDip_MarHE_PAM_infile.xlsx - PAM Fv/Fm values; for details refer to Iakovleva et al. (2024)

PocPorDip_MarHE_prelim_temprespcurve.pdf - colony-specific log-logistic temperature-Fv/Fm dose-response curves

PocPorDip_MarHE_summaryEDs_df.csv - ED5, ED50, ED95 with SD, SE, CI for all species/condition groups

PocPorDip_MarHE_temprespcurve.pdf - species/condition group log-logistic temperature-Fv/Fm dose-response curves with indicated ED5, ED50, ED95 and 95% CI

PocPorDip_MarHE_CollectionDepth.xlsx - Depth of sampled colonies

PocPorDip_MarHE_UW_colony.zip - Underwater photos of sampled coral colonies

Pilot_PocPor_MarHE.zip - Pilot CBASS run to inform CBASS heat-hold temperatures.

MarHE-CBASS-workshop_BenthicSurveyData.xlsx - CoralNet annotations file