We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in th
Data from the manuscript: Carnicer M, Canelas AB, Ten Pierick A, et al. Development of quantitative metabolomics for Pichia pastoris. Metabolomics. 2012;8(2):284-298. doi:10.1007/s11306-011-0308-1
GoldenPiCS modules and plasmids. Modules and plasmids are listed with corresponding cloning- and fusion sites and full sequences. DNA orientation is 5’to 3′. All plasmids are available at Addgene. (XL