Astrocyte knockdown of Drosophila Nkt selectively decreases night sleep

Figure. Nkt knockdown in fly astrocytes decreases night sleep. A) Expression patterns of two different astrocyte GAL4 drivers: eaat1-GAL4 and GMR86E01-GAL4. Both have been described as astrocyte drivers, although eaat1-GAL4 expresses in some cortex glia and in a small number of optic lobe neurons. Elav-GAL80 (eG80) was included in the background of eaat1-GAL4 flies to inhibit expression of Nkt.IR in neurons. B-C) eaat1-GAL4>Nkt.IR; eG80, GMR86E01-GAL4>Nkt.IR, and control populations. n=16-23 for all genotypes. GMR86E01-GAL4 was recently described by Kremer et al. (2017) as a good reference line for astrocyte expression. OL, optic lobe; Pr, protocerebrum. Size bars in A are 20 um. **, p<0.01 compared to controls.

图. 飞行星形胶质细胞中Nkt敲低降低夜间睡眠。A) 两种不同的星形胶质细胞GAL4驱动基因的表达模式：eaat1-GAL4和GMR86E01-GAL4。两者均被描述为星形胶质细胞驱动基因，尽管eaat1-GAL4在部分皮质胶质细胞以及少量视叶神经元中表达。Elav-GAL80（eG80）被纳入eaat1-GAL4果蝇的背景中，以抑制神经元中Nkt.IR的表达。B-C) eaat1-GAL4>Nkt.IR；eG80，GMR86E01-GAL4>Nkt.IR，以及对照组。所有基因型的n=16-23。GMR86E01-GAL4最近被Kremer等人（2017年）描述为星形胶质细胞表达的优良参考系。OL，视叶；Pr，原脑。图A中的尺寸条为20微米。**, 与对照组相比，p<0.01。