Dynamic redistribution of CTCF demarcates bifurcation of cytotoxic effector and memory precursor programs [ATAC-Seq]

CTCF is known to mediate long range chromatin intearction and 3D genome organization. In CD8+ T cells responding to actue infection, CTCF is redistributed to induce the the cytotoxic program while using its insulation function to suppresss memory precursor fate. Overall design: Naive P14 TCR transgenic, hCD2-Cre, Rosa26-lsl-GFP, Ctcf wild-type or floxed CD8+ T cells were adoptively transferred into CD45 disparate recipients followed by infection with LCMV Armstrong. On day 4 post infection, P14 cells were sort purified and used for ATAC-seq analysis along with naive cells.