Intestinal organoids derived from piglets were treated with the cell-free supernatant of Bacillus strain 541, and transcriptomic analysis was subsequently performed on three untreated and three treate
aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing