Kinetics of Membrane Charging and Its Dependence on Neuron Shape and Alignment with the Electric Field

This experimental series aimed to visualize and characterize plasma membrane (PM) polarization dynamics in hippocampal neurons exposed to 2 µs electric pulses, focusing on the influence of neuronal shape and position within the applied electric field. Neurons were pre-loaded with FluoVolt potentiometric dye, and pulsed laser strobe microscopy was employed to capture changes in membrane polarization. This study was partially supported by the NIH Brain Initiative (NEI R21EY034803).