Anatomical Structures, Cell Types, plus Biomarkers (ASCT+B) table for Lymph Node, v1.4

Anatomical Structures, Cell Types, plus Biomarkers (ASCT+B) tables (https://humanatlas.io/asctb-tables) aim to capture<br>the nested lymph-node structure of anatomical human body parts, the typology of cells, and biomarkers used to identify<br>cell types. The tables are authored and reviewed by an international team of experts. The lymph node ASCT+B tables are<br>derived from published literature, public datasets, and domain expertise from the authors. The table provides a<br>reference for a canonical human mesenteric lymph node; however, it is anticipated that these tables will be amended to<br>reflect lymph nodes draining other sites of the human body and changes arising from inflammation and other<br>perturbations. The gross anatomy of the lymph node was highly influenced by the following text: [Willard-Mack 2006]<br>(https://doi.org/10.1080/01926230600867727). Cell types and gene biomarkers derived from sequencing studies were<br>derived from the following studies: [James et al. 2020] (https://doi.org/10.1038/s41590-020-0602-z), [Xiang et al.<br>2020] (https://doi.org/10.3389/fcvm.2020.00052), and [Szabo et al. 2019] (https://doi.org/10.1038/s41467-019-12464-3).<br>Gene biomarkers for stromal cells were obtained from an orthogonal study in mice ([Rodda et al. 2018]<br>(https://doi.org/10.1016/j.immuni.2018.04.006)). Several studies employing flow cytometry and immunohistochemistry<br>aided in the identification of protein biomarkers. To support granular phenotyping, we list all reported biomarkers<br>including negative and differentially expressed proteins. We anticipate further refinement from multimodal studies<br>that pair sequencing and imaging studies from the same human sample. In total, this table reports 34 anatomical<br>structures, 45 cell types, and 223<br>biomarkers. <br> <br>**Bibliography:** <br><br>* James, Kylie R., Tomas Gomes, Rasa Elmentaite, Nitin Kumar, Emily L. Gulliver, Hamish W. King, Mark D. Stares, et al. 2020. “Distinct Microbial and Immune Niches of the Human Colon.” *Nature Immunology* 21 (3): 343–53. https://doi.org/10.1038/s41590-020-0602-z.<br>* Willard-Mack, Cynthia L. 2006. “Normal Structure, Function, and Histology of Lymph Nodes.” *Toxicologic Pathology* 34 (5): 409–24. https://doi.org/10.1080/01926230600867727.<br>* Xiang, Menglan, Rubén Adrián Grosso, Akira Takeda, Junliang Pan, Tove Bekkhus, Kevin Brulois, Denis Dermadi, et al. 2020. “A Single-Cell Transcriptional Roadmap of the Mouse and Human Lymph Node Lymphatic Vasculature.” *Frontiers in Cardiovascular Medicine* 7 (April). https://doi.org/10.3389/fcvm.2020.00052.<br>* Szabo, Peter A., Hanna Mendes Levitin, Michelle Miron, Mark E. Snyder, Takashi Senda, Jinzhou Yuan, Yim Ling Cheng, et al. 2019. “Single-Cell Transcriptomics of Human T Cells Reveals Tissue and Activation Signatures in Health and Disease.” *Nature Communications* 10 (1): 4706. https://doi.org/10.1038/s41467-019-12464-3.<br>* Rodda, Lauren B., Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, Sanjiv A. Luther, Ben A. Barres, Andrew D. Luster, Chun Jimmie Ye, and Jason G. Cyster. 2018. “Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity.” *Immunity* 48 (5): 1014-1028.e6. https://doi.org/10.1016/j.immuni.2018.04.006.