Gene expression profiles of active and restricted R/G-HIV+ primary human fetal astrocytes

The gene expression patterns of astrocyte harboring active vs restricted LTRs were similar, and that the active population demonstrated more widespread and robust changes. Overall design: Infection of astrocytes with VSVG pseudotyped Red/Green-HIV-1 wildtype and integrase (D116A) mutant. Astrocytes were sorted into latent (R+/G-), active (R+/G+), D116A (R-/G-) and untreated two weeks post-infection. RNA was isolated and sequenced in three separate donors.