Characterisation of Platelet Releasate Proteome in Relapsing-Remitting Multiple Sclerosis Reveals Dysregulation of Inflammatory Signalling and Extracellular Vesicle Dynamics

Multiple Sclerosis is an inflammatory neurodegenerative disease characterised by blood-brain barrier dysfunction and leukocyte infiltration into the central nervous system. Platelets are best known for their contributions to haemostasis, however upon activation platelets release an abundance of soluble and vesicular-associated proteins, termed the platelet releasate (PR). This milieu contains numerous inflammatory and vasoactive proteins which function to attract leukocytes and alter endothelial permeability. Here, we aimed to characterise the PR of Relapsing-Remitting multiple sclerosis (RRMS) patients, previously characterized with respect to thrombin generation dynamics compared to healthy controls. We carried out LFQ proteomic profiling of the PR from 15 RRMS and 19 aged-matched healthy controls. The PR proteome identified 9 proteins increased and 16 proteins decreased in RRMS patients.