Data for: Plasticity in mosquito size and thermal tolerance across a latitudinal climate gradient

Variations in heat tolerance among populations can determine whether a species can cope with ongoing climate change. Such variation may be especially important for ectotherms whose body temperatures, and consequently, physiological processes, are regulated by external conditions. Additionally, differences in body size are often associated with latitudinal clines, thought to be driven by climate gradients. While studies have begun to explore variation in body size and heat tolerance within species, our understanding of these patterns across large spatial scales, particularly regarding the roles of plasticity and genetic differences, remains incomplete. Here, we examine body size, as measured by wing length, and thermal tolerance, as measured by the time to immobilization at high temperatures (“thermal knockdown”), in populations of the mosquito Aedes sierrensis collected from across a large latitudinal climate gradient spanning 1300 km (34-44 °N). We find that mosquitoes collected from l..., This data on body size and thermal tolerance was collected by measuring the wing length and knockdown time of 13 populations of Aedes sierrensis across the Pacific Coast of North America. We also analyze a subset of the data from another experiment of 9 populations of laboratory-reared Aedes sierrensis from Couper et al. 2024 https://doi.org/10.1098/rspb.2023.2457., , # Data for: Plasticity in mosquito size and thermal tolerance across a latitudinal climate gradient

[https://doi.org/10.5061/dryad.x69p8czr9](https://doi.org/10.5061/dryad.x69p8czr9)

The first dataset KD-lat.lon.csv includes information about the site locations including latitude, longitude, and mean annual temperature. The next dataset KD-time.wings.analysis.csv includes information from the main plasticity experiment in which we measured knockdown time, wing length, and thorax length. The final dataset lab_reared.csv is a subset of data from the experiment in Couper et al. 2024 on the size of individuals from 9 other locations reared in the lab at 13, 17, 24, or 28°C.

## Description of the data and file structure

Use the code KD_analysis_submission_R2.R which pulls in the three datasets described in detail below to create the figures in the manuscript.

NA means that the measure was unavailable likely due to the thorax being damaged or that the measure was not relevant for that i...