Effect of organic fraction of municipal solid waste (OFMSW)-based biochar on purple. soil metagenome

Used as a soil amendment, this biochar may alter N transformation in scantily-N purple soil by changing the soil properties and microbial community structure. In the effort to investigate the effects of OFMSW-based biochar on bacterial community structure, soil characteristics, especially nitrogen condition, a 12-month pot incubation experiment was conducted. A purple soil in Chongqing, China,was amended by biochar which derived from OFMSW and was pyrolyzed at 700?C.Four treatments were built based on various biochar application rates which are 0%,1%, 3% and 5% w/w and were named as 0%BC, 1%BC, 3%BC and 5%BC. Severalphysicochemical properties of soil samples were monitored. Simultaneously, the DNA extracted form soil samples were sequenced on MiSeq platform to identify the bacterial community structure.