Sus scrofa Raw sequence reads

The adult Leydig cells (ALCs), originated from stem Leydig cells (SLCs), can secrete testosterone which is essential for germ cell development and sexual behavior maintenance. Ethane dimethane sulphonate (EDS), a well-known alkylating agent, has been reported to specifically ablate ALCs. In this study, the primary isolated pig LCs (containing SLCs and differentiated LCs) and EDS-treated LCs (almost exclusively SLCs) were collected for RNA-seq. The difference between the primary group and the EDS-treated group can reflect the expression spectrum of mRNAs and miRNAs related to pig LCs cell lineage differentiation, which can provide a basis for subsequent studies.