Seedling_EM_dat.csv

We grew ectomycorrhizal (EM) tree seedlings <i>Tsuga canadensis, </i><i>Pinus strobus, </i>and <i>Quercus </i>spp. for two years across three secondary forests dominated by arbuscular mycorrhizal (AM) <i>Acer </i>spp. trees in New York, USA. Seedlings were planted next to either the dominant AM <i>Acer </i>spp. or scattered EM <i>Betula </i>spp. A subset of seedlings received soil inoculum from local EM dominated forests, which included an old-growth <i>Tsuga</i>-hardwood forest, residual <i>Tsuga</i>-hardwood forest, as well as a secondary forest dominated by <i>Quercus rubra </i>and planted <i>Picea abies. </i>Seedling survival and height growth were monitored annually, and upon harvest we measured seedling shoot mass and EM fungal colonization of roots. We identified EM fungi using morphotyping following by restriction fragment length polymorphism (RFLP) of rDNA internal transcribed spacer (ITS) amplicons and Sanger DNA sequencing of RFLP exemplars.