X-Ray Diffraction data from BOVINE TRYPSIN, source of 5F6M structure
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http://data.sbgrid.org/dataset/201
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Trypsin crystals were obtained according to the method of Liebschner et.al (Liebschner et al., 2013). Lyophilized bovine pancreas trypsin was purchased from Sigma-Aldrich (T1005) and dissolved at a concentration of 30 mg/mL into 30mM HEPES pH 7.0, 5 mg/mL benzamidine and 3mM CaCl2. Crystals were obtained from a solution of 200mM Ammonium sulfate, 100mM Na cacodylate pH 6.5, 20% PEG 8000 and 15% glycerol. Data collected for simultaneous Bragg and Diffuse Scattering processing.
提供机构:
SBGrid Data Bank
创建时间:
2015-12-18



