Experimental evolution of copepods during adaptation to heat stress

This sequencing project was intended to quantify changes in allele frequencies during adaptation to high temperature environments in the copepods Tigriopus californicus. Hybrids between a heat-tolerant population (SD: San Diego, California) and a heat-sensitive population (SH: Strawberry Hill Oregon) were subjected to either a high temperature selective regime or a control regime (no heat stress) one a generation for 12 generations. For each treatment, 4-6 independent replicate populations were maintained in parallele. Also, the experiment was conducted for each reciprocal cross (SDfxSDm and SHfxSDm) After reaching the 12th generation, life-history and metabolic phenotypes were quantified, and whole-genome sequencing performed on pools of ~100 individuals per replicate population.