Fate restricted stromal fibroblasts and adipocytes demonstrate multi-modal responses to tissue injury

Adult connective tissues rearrange themselves during injury, disease, and aging. This response is widely considered to involve cross-lineage conversion between two differentiated stromal cell types: fibroblasts and adipocytes. Here, using lineage tracing approach, we explore the transcriptome profiles of Adipoq-lineage positve mature adipocytes and En1-lineage postive fibroblasts in in vivo mouse skin wounds. The data presented here show that wound adipocytes and fibroblasts still possess distinct molecular signatures, and do not undergo cross-lineage conversion. Wound adipocytes and fibroblasts were FACS sorted as GFP+ cells from day 10 and day 21 wounds from AdipoqCre;R26mTmG and En1Cre;R26mTmG mice. Sorted cells were subjected to RNA-sequencing. Comparative gene expression profiling analysis of RNA-seq data of wound adipocytes and fibroblasts.