Gene expression profile (GEP) of K562 cells expressing wtCALR, CALRins5 and CALRdel52

In this work, we compared gene expression profile (GEP) of K562 cells transduced with the retroviral vector LCALRins5IDN or LCALRdel52IDN with K562 cells transduced with LwtCALRIDN In order to unravel MPL-independent mechanisms underlying the effect of CALR mutations on MPN pathogenesis, we analysed the transcriptional changes induced by the CALRins5 or CALRdel52 overexpression in K562 cells, which lack MPL expression In a set of three independent experiments, K562 cells were transduced with the LCALRwtIDN (CALR wild-type, CALRwt), LCALRdel52IDN (CALRdel52), LCALRins5IDN (CALRins5) retroviral vectors. After NGFR-based purification, the transduced cells were profiled by means of Affymetrix U219 Array