Effect of the FGFR kinase inhibitors AZD4547 and BGJ398 on gene expression in HaCaT cells

Fibroblast growth factors (FGF) play important roles during embryonic development as well as in the adult organism in tissue homeostasis, regeneration, repair and metabolism. We recently showed that inhibition of FGF receptor (FGFR) signaling in keratinocytes either pharmacologically or in a mouse FGFR1/2 knockout model, promoted expression of interferon-stimulated genes (ISGs) (Maddaluno et al, 2020). Here we analyzed the transcriptome of human HaCaT keratinocytes in response to treatment with the FGFR kinase inhibitors AZD4547 and BGJ398. HaCaT cells were cultured to confluency in DMEM (10% FBS), then treated for 5 h in DMEM (5% FBS) with either vehicle (DMSO), 10 µM AZD4547 or 3.6 µM BGJ398. contributor: Functional Genomics Center Zürich