Richard Wheeler (2010) CIL:10602, Leishmania mexicana, Mus musculus, macrophage, parasite. CIL. Dataset

Macrophage cell line cell infected with Leishmania mexicana. Five amastigotes can be seen within a single large parasitophorous vacuole in the macrophage. Raw 264.7 cells (a leukaemic mouse macrophage line) were infected with amastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) at an MOI of 10 and maintained in RPMI for 4 days. Cells were fixed with 2% paraformaldehyde, and DNA was labelled with propidium iodide following incubation with 50 ug/ml RNaseA for 30 min at room temperature. Z stack of 93 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is a phase channel image that is not currently displayed, but can be turned on in the full viewer mode.

巨噬细胞系细胞感染利什曼原虫墨西哥亚种。在单个大型寄生物泡内可见五个前鞭毛虫。采用小鼠白血病巨噬细胞系（Raw 264.7细胞）感染利什曼原虫无鞭毛体形态（世界卫生组织菌株MNYC/BZ/62/M379），感染复数（MOI）为10，并在RPMI培养基中维持培养4天。细胞用2%多聚甲醛固定，并在室温下与50 µg/ml RNaseA孵育30分钟后，用碘化丙啶（propidium iodide）标记DNA。使用Leica DM5500 B型共聚焦荧光显微镜（配备100x/1.4 NA物镜）和Hamamatsu冷却CCD相机（Orca）捕获的93张图像Z栈，Z间距为0.194微米。请注意，存在一个相位通道图像当前未显示，但在完整查看模式下可以打开。