Composition of fecal microbiota from mice fed with diets containing different manganese doses Raw sequence reads

We investigated whether dietary Mn alters microbiota composition. To ensure a uniform and normalized microbiota composition, we first cohoused 4-week-old mice by feeding Mn-adequate diet (35 mg/kg Mn) for 28 days. Then we fed mice either a Mn-deficient (<0.01 mg/kg Mn), Mn-adequate (35 mg/kg Mn), or Mn-supplemented (300 mg/kg Mn) diet for 14 days. We examined the effect of dietary Mn on the gut microbiota by collecting feces at the baseline (Day 0) and the end of the Mn manipulation (Day 14) and determining the microbiota composition by Illumina 16S rRNA gene sequencing.