Effects of IDH1-R132H on mouse neural stem cells. Mus musculus

Mouse neural stem cells were generated from conditional knock-in mice. Mutant IDH1 is conditionally expressed following expression of Cre-recombinase. Cre-recombinase was incorporated in vitro via adenoviral-Cre recombinase. Addtionally, adenoviral-GFP was also used to control for effects of adenoviral infection. D-2HG, the metabolic product of IDH1-R132H, was detected in cell lines post-cre transduction Overall design: A total of three cell lines were used for this study. Each of the three cell lines were transduced with either adenoviral-GFP (Control) or adenoviral-Cre recombinase (induces expression of mutant IDH).