Expression data from H2A.Z isoforms single KO cells

The histone variant H2A.Z is evolutionarily conserved from yeast to vertebrates. H2A.Z regulates gene expression when localized to promoter region. Recently, we identified two genes encoding H2A.Z, H2A.Z-1 and H2A.Z-2 in vertebrate genome. However, it is not clear that both H2A.Z-1 and H2A.Z-2 were required for the function of H2A.Z in gene regulation. To address this issue, we generated the H2A.Z-1 and H2A.Z-2 single knockout cells in chicken DT40 cells.We used microarrays to analysis the alternation of gene expression profiles in H2A.Z-1 KO and H2A.Z-2 KO cells and identify classes of up or down regulated gene by H2A.Z-1 and H2A.Z-2. In H2A.Z-2 KO cells, H2A.Z-2 gene is knocked out constitutively. However, in H2A.Z-1 KO cells, H2A.Z-1 gene is conditionally knocked out by tetracycline. The expression of H2A.Z-1 transgene is suppressed completely when H2A.Z-1 KO cells were cultured with tetracycline for three days. For this reason, we prepared the total RNA of H2A.Z-1 KO cells and H2A.Z-2 KO cells treated with tetracycline for three days and hybridization on Affymetrix microarrays.