Comparative RNAseq of Mouse Parp3+/+ and Parp3-/- Neural Stem Progenitor Cells (NPSC)

To identify the biological pathways underlying impaired differentiation of Parp3-deficient NPSC to astrocytes, RNAseq of the Parp3+/+ and Parp3-/- astrocytes were performed 4 days upon differentiation. Briefly, NPSC were isolated from the forebrains of Parp3+/+ and Parp3-/- 8-day-old mice. Viable single cells were induced to differentiate into astrocytes for 4 days and samples were collected for total RNA isolation and RNA sequencing. The experiment includes 3 independent differentiations (biological replicates) of Parp3+/+ and Parp3-/- NPSC to 4 days astrocytes. The samples represent 3 Parp3+/+ 4d-astrocytes and 3 Parp3-/- 4d-astrocytes.