TARGET-seq+ metadata

Metadata for TARGET-seq+ single-cell whole transcriptome data from bone marrow samples from 9 cases with clonal hematopoiesis and 4 control samples. For details on cell sorting and the TARGET-seq+ protocol see the methods section of the manuscript. Cells are named with their plate and well IDs and the subject ID. Empty wells (no-cell controls) are named "blank".
Column descriptionsColumn 1 ('Cell'): Cell ID
Column 2 ('Sample'): Donor ID
Column 3 ('Sex'): Donor sex
Column 4 ('Age'): Donor age
Column 5 ('Sample_type'): Type of sample; CH = clonal haematopoiesis sample; WT_control = non-CH wild-type control sample; No_template_control = empty well control
Column 6 ('Sort'): FACS sort ID
Column 7 ('Plate'): = Plate ID
Columns 8-24: FACS indexing expression profiles.
Column 25 ('Single_cell'): indicates whether single cell sorted into well; FALSE indicates wells with more than one cell (excluded from analysis)
Columns 26-36: FACS profiles and immunophenotypic population based on gating on FACS indexing expression values
Column 37 ('Genotype'): Clonal identity assigned based on single-cell genotyping
Column 38 ('Cluster'): Cell type based on transcriptome clustering
Column 39 ('PassedQC'): Whether the cell passed transcriptome quality control