Nociceptor neurons control pollution-mediated neutrophilic asthma

The immune and sensory nervous systems, having evolved in parallel, communicate through shared receptors and transmitters to maintain homeostasis and respond to both external and internal disruptions. Although neural responses often confer protective benefits, they can also exacerbate inflammation during allergic reactions such as asthma. In our study, we modeled pollution-exacerbated asthma by exposing mice to ambient PM2.5 particles alongside ovalbumin. Compared to exposure to ovalbumin alone, this co-exposure significantly increased the numbers of neutrophils and γδ T cells in bronchoalveolar lavage fluid and lung tissue, respectively. We found that silencing nociceptor neurons at the peak of inflammation using intranasal QX-314 or ablating Trpv1-expressing neurons reduced lung neutrophil accumulation. Live in vivo intravital imaging confirmed that neuronal ablation reduced neutrophil numbers and increased their net displacement capacity. In neurons isolated from mice with pollution-..., Animals. All procedures involving animals adhered to the guidelines of the Canadian Council on Animal Care (CCAC), McGill University (Mgcl-8184), and the Queen's University Animal Care Committee (UACC, protocol 2384). Mice were housed in individually ventilated cages with free access to water and food under 12-hour light cycles. 
Parental strains C57BL/6 (# 000664), Dtafl/fl (# 010527, # 009669), tdTomatofl/fl (# 007914), Trpv1cre/cre (# 017769), and Scn10acre/cre (# 036564) were purchased from The Jackson Laboratory. Male and female mice were bred in-house and used at 6–12 weeks of age. Crosses were performed to generate the following genotypes: Scn10acre/wt::Dtafl/wt, Trpv1cre/wt::Dtafl/wt, Trpv1cre/wt::tdTomatofl/wt, and littermate control, namely: Scn10awt/wt::Dtafl/wt, TRPV1wt/wt::Dtafl/wt. Male and female progeny mice were used between 8 and 16 weeks of age.
Ovalbumin Model of Allergic Airway Inflammation. On days 0 and 7, mice were sensitiz..., # Data from: Nociceptor neurons control pollution-mediated neutrophilic asthma

Dataset DOI: [10.5061/dryad.hhmgqnkwq](https://doi.org/10.5061/dryad.hhmgqnkwq)

## Description of the data and file structure

All animal procedures followed CCAC, McGill, and Queen’s University guidelines. Mice (C57BL/6 and transgenic strains) were bred in-house and used at 6–16 weeks. Allergic airway inflammation was induced using an ovalbumin (OVA) sensitization/challenge model ± particulate matter, with optional neuronal silencing (QX-314).

Samples (BALF, lung, neurons) were collected for flow cytometry, ELISA, qPCR, imaging, and RNA-seq. Additional assays included macrophage/neuron cultures, calcium imaging, and intravital microscopy. RNA-seq and in silico analyses (mouse and human datasets) were performed using standard pipelines.

Statistics used t-tests/ANOVA (p ≤ 0.05). Replicates represent independent animals or samples; one RNA-seq outlier was excluded.

#### File name: Wang._eLife._Raw_data.xls..., ,