Text S1 - Repurposed Automated Handheld Counter as a Point-of-Care Tool to Identify Individuals ‘At Risk’ of Serious Post-Ivermectin Encephalopathy

Supplemental Figures. Figure S1 in Text S1: Microfilaria in 60 µm sensor. Center image showing the 60 µm sensor with the various ‘visible’ parts labeled 1–5 in order of the sample flowing through the microfluidic sensor. 1- Mesh to filter clumps and large particles that would block the aperture. Phase contrast microscopic images of filter focused in front of the filter (1A) and behind the filter (1B). 2- The aperture area, with phase contrast images of the triangular segment at 10x magnification (2A) and 40x magnification (2B); and 40X magnification of the banded area (2C). Microfilariae after passing through (3) and just before the instrument beeps (4) Accumulation of mf at the end of the microfluidic chamber (5A & 5B). Figure S2 in Text S1: Volume of blood sample. Histograms on the top correspond to B. malayi infected cat blood tested at various volumes (50 µl to 5 µl, in intervals of 5 µl). The HHAC counts corrected for the dilution factor are tabulated. M1 denotes the peak on the histogram. Figure S3 in Text S1: Size based differentiation capability of Scepter. Overlapping histograms showing the gates of Brugia malayi (O1, violet), Loa loa (O2-field, red; O3-lab, orange), Dirofilaria immitis (O4, blue) compared to normal human blood (O5, grey). The O5 gate corresponds to all the cells in the blood (minus RBC). (DOCX)