Primer pairs used for mutagenesis of CP-encoding sequence of PVY<sup>N</sup> or PVY<sup>O</sup>.
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aRestriction sites are in italic and underlined. The nucleotides introduced before the restriction sites increase the efficiency of digestion. Mutated nucleotides are in bold type. “P” indicates that the 5′-terminus of the primer is phosphorylated. Tm indicates melting temperature in Celsius degrees. Primer pairs used for mutagenesis of CP-encoding sequence of PVYN or PVYO.
创建时间：
2014-12-26
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