16S rRNA gene amplicon sequencing of a karstified aquifer bacteria culture

Removal of nitrate by microbial denitrification in freshwater aquifers is often limited by low concentrations of organic carbon. In these nutrient-poor ecosystems nitrate-reducing bacteria that can use inorganic compounds such as Fe(II) (NRFeOx) as electron donors could play a major role in nitrate degradation. Microbial communities for enrichment cultures were passively sampled (MTDs) and transferred to low phosphate medium (LPM) (Hegler et al. 2008) amended with 2 mM of NaNO3 and 2 mM of FeCl2. Enrichments were incubated at room temperature in the dark and transferred to fresh medium every 2-3 weeks (for about a year in total) after complete oxidation of iron(II). Conditions were always kept anoxic. This dataset represents the raw sequencing files of nearly full length 16S rRNA amplicon sequencing of the microbial community composition of a novel truly autotrophic NRFeOx enrichment culture.