A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene. Yr7 yellow rust resistance gene mapping in wheat

We mapped the yellow rust resistance gene Yr7 to a low-recombination region of chromosome 2B in wheat. We used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.