RNA samples of human peripheral blood mononuclear cells for Methylated RNA immunoprecipitation-seq

We established m6A modification profiles using MeRIP-seq in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients and controls (HC), and investigated m6A-related lncRNAs in SLE for novel potential roles in SLE. Compared with controls, m6A level was lower in SLE patients,426 lncRNAs and 2,331 mRNAs were differentially expressed in SLE patients. Overall design: RNA samples of PBMCs from three new-onset SLE patients and three matched controls (HC) were utilized for Methylated RNA immunoprecipitation-seq (MeRIP-seq)