Result-X101SC25013530_Z02_J001_B1_43

This dataset contains quantitative proteomic profiles of bovine cumulus cells collected at key stages of in vitro oocyte maturation. The purpose of this study was to investigate the dynamic protein expression changes that occur in cumulus cells during the transition from the germinal vesicle (GV) stage to the metaphase II (MII) stage, providing insight into the molecular mechanisms that support oocyte maturation.

Cumulus–oocyte complexes (COCs) were aspirated from bovine ovaries and cultured under standard in vitro maturation (IVM) conditions. Cumulus cells were isolated at GV (0 h) and MII (24 h) time points. Proteomic analysis was performed using high-resolution liquid chromatography–tandem mass spectrometry (LC–MS/MS). Protein identification and quantification were achieved through database-dependent searches, and label-free quantification was applied to determine relative protein abundance across biological replicates.

The dataset includes raw mass spectrometry files, processed protein identification and quantification tables, and experimental metadata. These data provide a resource for understanding the proteomic remodeling of cumulus cells during oocyte maturation and may serve as a reference for studies on mammalian follicular development, oocyte competence, and assisted reproductive technologies.