Analysis of differential expression profiles of Long noncoding RNA in peripheral blood of patients with active rheumatoid arthritis

we studied the expression profile of lncRNA in peripheral blood of patients with active RA LncRNA and mRNA expression in peripheral blood from two activity RA patients and two healthy donors were analyzed with microarray profiling. peripheral blood of five active RA patients and five healthy control members were collected. Quantitative real-time PCR was further used to screen and verify the expression of lncRNA to verify the expression of the selected lncRNA; perform bioinformatics analysis to study the potential function of the differentially expressed genes, construct a receiver operating characteristic (ROC) curve to evaluate the clinical diagnostic value of the identified lncRNA, and use correlation analysis to explore the relationship between aberrantly expressed lncRNA that may have diagnostic value and clinical indicators. Two RA patients were used as the RA group, and two healthy people were used as the control group, and 6 ml of peripheral blood were collected respectively.,then perform LncRNAs chips by Arraystar Human LncRNAs chip (Arraystar)