CIL:50451, Mus musculus, Mouse brain tissue. In Cell Image Library
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Brief description of sample preparation
After cryofixation by high-pressure freezing and freeze-substitution, cryofixedmouse brain slices were rehydrated gradually. Rehydrated samples were then imaged with confocal microscopy to capture the DRAQ5 and tdTomato signals. Next, the samples were stained using a high-contrast en bloc staining protocol. Then the samples were dehydrated for resin infiltration and embedding, followed by imaging with X-ray microscopy and then SBEM.
Serial Block-face Scanning Electron Microscopy
The mouse brain slice wasimaged with a Merlin scanning electron microscope equipped with a 3View2XP and OnPoint backscatter detector. The volume was collected at 2 kV, with 6.8 nm pixels; 70 nm Z steps and 15% local gas injection. The raster size was 10k x 15k and the Z dimension was 659 sections. The volume was aligned using cross correlation and visualized using IMOD.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
