Data packages related to "MF1 kills liver cancer cells by jointly regulating autophagy and ferroptosis"

The Cell Counting Kit-8 (CCK-8) assay was used to assess the viability of HepG2 cells, and the IC50 was obtained after treatment with MF1. The degree of lipid peroxidation in HepG2 cells was measured by malondialdehyde (MDA) assay. Apoptosis, intracellular ROS, and GSH/GSSG levels were determined using corresponding kits. The redox state of iron ions in HepG2 cells after MF1 treatment was also examined. Electron microscopy was used to observe the morphology of HepG2 cells after treatment. Subsequently, recovery experiments were conducted by intervening with the ferroptosis inhibitor Liproxstatin-1 and the autophagy inhibitor 3-MA on HepG2 cells stimulated with MF1.The therapeutic targets of MF1 were predicted using network pharmacology and further validated through molecular docking and RT-qPCR.