A bioprinted model of pregnant human uterine myometrium

We have utilized primary human uterine smooth muscle cells to bioprint myometrial tissue rings containing >75% viable cells with elongated, smooth muscle morphology. Immunofluorescence confirmed expression of smooth muscle markers (caldesmon, alpha smooth muscle actin, and smooth muscle myosin), contractile-associated proteins (oxytocin receptor, prostaglandin receptors and connexin-43), and steroid hormone receptors (estrogen and progesterone receptors) characteristic of pregnant human uterine myometrium. Bioprinted tissue ring area contracted in response to physiological agonists oxytocin (p<0.001), prostaglandin F2 alpha (p=0.003), and prostaglandin E2 (p<0.001), and relaxed in response to the nitric oxide donor S-nitrosoglutathione (p=0.0004). , , # A bioprinted model of pregnant human uterine myometrium

Dataset DOI: [10.5061/dryad.gf1vhhn0m](10.5061/dryad.gf1vhhn0m)

## Description of the data and file structure

Total proteomic profiles (n=3 for each group) of parent myometrial tissue, primary uterine smooth muscle cells grown in 2-dimensional culture, and synthetic myometrial tissues were collected and analyzed via TMT mass tag quantitative proteomics

### Files and variables

#### File: TMT\_Proteomics\_Primary\_vs\_Parent\_Tissue.txt.xlsx

**Description:** Tandem Mass Tag Quantitative Proteomic Analysis of Primary Cells vs Parent Tissue

##### Variables

* **Column A (ID)– UNIPROT ID**
* **Column B (Description)- Protein Description**
* **Column C (CoveragePerc)- Percentage of protein coverage mapped**
* **Column D (NumPep)- Number of Peptides Mapped**
* **Column E (NumPSMSs)- Number of Peptide Scoring Matches Mapped**
* **Column F (NumUniqPep)- Number of Unique Peptides Mapped**
* **Column G (imput)- Where Imputed Peptides...,