Metagenomics of a consortium from rhizosphere of Capsicum chinense plants

The objective was to describe the composition of a consortium isolated from the rhizome of Capsicum chinense. To analyze the microbial consortium, samples from the rhizosphere of C. chinense plants with wilt symptoms. The consortium was maintained in V8 agar (Medina & Platt 1999). DNA extraction was done with ZymoBIOMICS DNA Microprep Kit (Zimo Research, https://www.zymoresearch.com/collections/zymobiomics-dna-kits). Analysis ITSs or 16S amplicons were sequenced (with replicate) MiSeq format 2x300, to obtain 300,000 PE readings by sample. Quality check of fastq files was performed with FastQC, then bioinformatics analyses were processed using readpipeline and MG_RAST (http://www.mg-rast.org/index.html)