Expression data from Healty Control and XP-A melanocytes. Expression data from Healty Control and XP-A melanocytes

Xeroderma Pigmentosum (XP) is a DNA repair disorder characterized by photosensitivity, resulting in occurrence of freckle-like pigmented maculae and depigmented maculae on sun-exposed areas. XP complementation group A (XP-A) is the most frequent type in Japan, and patients with XP-A present most severe cutaneous and neurological symptoms due to nucleotide excision repair deficiency. Here, we established induced pluripotent stem cells (iPSCs) derived from XP-A patients and successfully differentiated into melanocytes. To elucidate the pathophysiology of XP, we comprehensively analyzed the difference in gene expression between XP-A-iMCs and healthy-control-iPSC-derived melanocytes (HC-iMCs) 4 hours and 12 hours after irradiation with 30 J/m2 or 150 J/m2 of UV-B using microarray analysis. Overall design: HC-iMCs, XP-A-iMCs and NHEM were irradiated with low dose (30 J/m2) or high dose (150 J/m2) of UV-B, followed by incubation for 4 hours or 12 hours. Total RNA was extracted from UV-B irradiated and pre-irradiated samples.