Characteristics of HBxAg and miR-148a in tumor and nontumor compartments from HBV-infected HCC patients.

a. Sex 1  =  male; 2  =  female.
b. Hepatitis b surface antigen (HBsAg) presence in serum: 0  =  no, 1  =  yes.
c. NT  =  non-tumor; T  =  tumor.
d. Intensity of HBxAg staining was evaluated as 0 (no signal) through 3 (intense signal).
e. Tissue distribution of HBxAg: S  =  scattered, L  =  lobular, D  =  diffuse.
f. Negative ΔΔCt value  =  elevated expression of miR-148a in tumor; positive ΔΔCt value  =  elevated expression of miR-148a in non-tumor liver.
g. Fold change is calculated as 2ΔΔCt. The average fold change for elevated miR-148a was calculated by adding up the fold-change from each patient with a positive ΔΔCt value divided by the number of patients with a positive ΔΔCt value. The positive ΔΔCt values listed in this table are plotted in Figure 1 and represent elevated miR-148a levels in liver compared to tumor. Parallel calculations were performed to determine the average fold change among patients with negative ΔΔCt values, which are plotted in Figure 1 as elevated miR-148a levels in tumor compared to liver.