A new species of Periglandula symbiotic with the morning glory Ipomoea tricolor

Many morning glories in the family Convolvulaceae contain ergot alkaloids, which are important agricultural and pharmaceutical compounds produced exclusively by fungi. In the few morning glories that have been investigated in detail, the ergot alkaloids are associated with the presence of a symbiotic fungus from the family Clavicipitaceae. The genus Periglandula was erected in the family Clavicipitaceae in 2011 for two species of morning glory symbionts. Biochemical and limited sequence data indicate that Ipomoea tricolor, a commonly cultivated morning glory from Mexico, contains a Periglandula species but the fungus is uncharacterized, and no signs of it have ever been detected in the plant. Our goal was to isolate and characterize this fungus. We isolated the fungus in pure culture, characterized it morphologically, and prepared sufficient DNA from it to sequence its genome. Phylogenetic analyses based on multiple genes indicate that the symbiont of I. tricolor is distinct from, but r..., The genome of the fungal symbiont of Ipomoea tricolor was sequenced (GenBank accession JBIMFC000000000) from DNA obtained from an axenic culture. Sequence reads were obtained by Illumina NextSeq technology at the West Virginia University and Marshall University Genomics Core facilities and assembled with SPAdes version 3.15.4. Homologs of the following loci were extracted from the genome of the symbiont of I. tricolor for phylogenetic analyses based on identity with homologs from the Periglandula ipomoeae genome: γ-actin (actG), β-tubulin (tubB), mitochondrial ATPase 6 (atp6), largest subunit of RNA polymerase II (rpbA), dimethylallyltryptophan synthase (dmaW), and the ribosomal internal transcribed spacer region (ITS). Sequences were aligned by MUSCLE as found in MEGA X (Kumar et al. 2018) and trimmed by eye. Maximum likelihood analyses with 1000 bootstrap replications were performed on ITS, dmaW, and concatenated actG, tubB, atp6, and rpbA loci. Those loci were selected for further an..., , # Alignments and sequence data supporting \"A new species of Periglandula symbiotic with the morning glory Ipomoea tricolor\"

[https://doi.org/10.5061/dryad.jdfn2z3mh](https://doi.org/10.5061/dryad.jdfn2z3mh)

## Description of the data and file structure

Data file contains DNA sequence alignments used as the foundations of phylogenetic trees appearing in Figure 3, 4, and 5 of Hazel and Panaccione, \"A new species of *Periglandula* symbiotic with the morning glory *Ipomoea tricolor.\"*  Data are in FASTA format and ready to be copied and pasted for phylogenetic analyses.  Title line for each sequence contains organism name, locus name, and GenBank accession number. The sole exception to this naming pattern is a concatenated, four-gene data set, in which case the alignment data are immediately followed by individual trimmed sequence data labeled with organism name, locus, name and GenBank accession number.  

### Files and variables

#### File: Hazel\_and\_Panaccione\_Alignments\_for\_Drya...,