fluorescent beads for 3D microscope quantification

To detect a set of fluorescent beads by flow cytometry to compare their relative fluorescence intensity to that measured by epifluorescence microscopy Conclusion: Analyses of fluorescence intensities of the beads by flow cytometry proved to be a sensitive, rapid and equally sensitive method as compared to epifluorescence microscopy TruCount beads (BD) were used as an internal standard to check for fluorescence intensities