Data S1 - Establishing Clonal Cell Lines with Endothelial-Like Potential from CD9hi, SSEA-1? Cells in Embryonic Stem Cell-Derived Embryoid Bodies

Microarray analysis of gene expression in RoSH-like cells vs ESCs. Total RNA (2 ��g) from each sample of E-RoSH2.1 p6 and p10, E-RoSH3.2 p2 and p7, RoSH2 p9 and p13, E-RoSHL1 p5 and p6, and E14 mESCs p15 and p16 cultures were converted to biotinylated cRNA using the Illumina RNA Amplification Kit (Ambion, Inc., Austin, TX) according to the manufacturer's instructions. Samples were purified using the RNeasy kit (Qiagen, Valencia, CA). Hybridization to the Sentrix Mouse Refseq-8 Expression BeadChip (Illumina, Inc., San Diego, CA), washing and scanning were performed according to the Illumina BeadStation 500x manual. The data were extracted, normalized and analyzed using Illumina BeadStudio provided by the manufacturer. (4.12 MB XLS)